Genetic depletion studies inform receptor usage by virulent hantaviruses in human endothelial cells
نویسندگان
چکیده
Hantaviruses are RNA viruses with known epidemic threat and potential for emergence. Several rodent-borne hantaviruses cause zoonoses accompanied by severe illness death. However, assessments of zoonotic risk the development countermeasures challenged our limited knowledge molecular mechanisms hantavirus infection, including identities cell entry receptors their roles in influencing viral host range virulence. Despite long-standing presumption that β3/β1-containing integrins major receptors, rigorous genetic loss-of-function evidence supporting requirement, decay-accelerating factor (DAF), is lacking. Here, we used CRISPR/Cas9 engineering to knockout candidate singly combination, a human endothelial line recapitulates properties primary microvascular cells, targets infection humans. The loss β3 integrin, β1 and/or DAF had little or no effect on large panel hantaviruses. By contrast, protocadherin-1, recently identified receptor some hantaviruses, substantially reduced infection. We conclude molecules necessary PCDH1-independent remain be discovered.
منابع مشابه
Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
Objective(s): This study was designed to investigate the effect of receptor for advanced glycation end products (RAGE), S100A12 and C-reactive protein (CRP) on the release of circulating endothelial cells (CECs) from human coronary artery endothelial cells (HCAECs). Materials and Methods: HCAECs were cultured in increasing concentration of CRP (0, 12.5, 25, 50μg/ml) or S100A12 protein (0, 4, 1...
متن کاملCapillary Network Formation by Endothelial Cells Differentiated from Human Bone Marrow Mesenchymal Stem Cells
Human bone marrow derived mesenchymal stem cells (HBMSCs) have the potential to differentiate into cells such as adipocyte, osteocyte, hepatocyte and endothelial cells. In this study, the differentiation of hBMSCs into endothelial like-cells was induced in presence of vascular endothelial growth factor (VEGF) and insulin-like growth factor (IGF-1). The differentiated endothelial cells were exam...
متن کاملEXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE GENE (iNOS) STIMULATED BY HYDROGEN PEROXIDE IN HUMAN ENDOTHELIAL CELLS
Inducible nitric oxide synthase (iNOS) gene expresses a calcium calmudolin-independent enzyme which can catalyse NO production from L-arginine. The induction of iNOS activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (LPS). Previous studies indicated that all nitric oxide synthases (NOS) activated in human umbilical vein endot...
متن کاملreceptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by c-reactive protein
objective(s): this study was designed to investigate the effect of receptor for advanced glycation end products (rage), s100a12 and c-reactive protein (crp) on the release of circulating endothelial cells (cecs) from human coronary artery endothelial cells (hcaecs). materials and methods: hcaecs were cultured in increasing concentration of crp (0, 12.5, 25, 50μg/ml) or s100a12 protein (0, 4, 10...
متن کاملAndrogen receptor in human endothelial cells.
Androgen receptor (AR) is a ligand-inducible transcription factor, and a member of the steroid-thyroid-retinoid receptor superfamily, that mediates the biological effects of androgens in a wide range of physiological and pathological processes. AR expression was identified in vascular cells nearly 20 years ago, and recent research has shown that AR mediates a variety of actions of androgens in ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: eLife
سال: 2021
ISSN: ['2050-084X']
DOI: https://doi.org/10.7554/elife.69708